数字化模拟文库筛选是指以发表的CNS以及相关高质量论文结果自建数据库、STRING数据库、BioGRID数据库、iDRBP-MMC数据库、JASPAR数据库等为数据基础，结合客户科研需求给出最适个性化项目方案，以客观互作和调控结果为基准，结合酵母双杂筛库和验证（Y2H）、双分子荧光互补（BiFC）、荧光素酶互补实验（LCA）、免疫共沉淀（Co-IP）、GST拉下实验（GST Pull-Down）、酵母单杂筛库和验证（Y1H）、双荧光素酶报告基因检测实验（Dual-Luciferase Reporter Assay）、凝胶阻滞实验（EMSA）、染色质免疫沉淀测序（ChIP-seq）等验证结果，全面解析蛋白/蛋白或者蛋白/核酸互作以及调控关系。

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服务流程

服务项目	客户提供	最终交付
数字化模拟文库筛选验证	基因序列信息	分析数据

文案展示

The Nucleome Data Bank: web-based resources to simulate and analyze the three-dimensional genome

期刊：Nucleic Acids Research

The .ndb file format. The .ndb format is designed to store data about the 3D conformation of genomes and contains enough information to be a sufficient input for physical simulations of chromosomes. The .ndb file format is inspired by the .pdb format and it is designed in strict analogy with it. The file contains 12 fields marked in figure by different colors. The first field describe the type of nuclear element: CHROM for a chromatin segment or DNAELE for any other biological element associated with DNA, whether this element is bound to DNA or free to diffuse in the nucleus. The second field is the sequential index associated to the nuclear element. The third field reports epigenetic information for the CHROM elements (chromatin type annotations in this case) or any two-letter identifier for DNAELE, TF, for a transcription factor, CH for cohesin protein complex, for example. The fourth field contains information about the DNA sequence, such as the presence of a CTCF-binding motif together with its orientation. Field number five labels the different molecular chains, e.g. 1P for the paternal copy of chromosome 1 and 1M for the maternal copy. Field number six contains a progressive index for the elements within their respective molecular chain. Fields seven, eight and nine are the spatial coordinates X, Y and Z of the nuclear element. Fields 10 and 11 represent the start and end genomic coordinate of the locus in the case of CHROM element. For DNA-bounds DNAELE elements fields 10 and 11 indicate the binding site, while a dash indicates that the element is unbound. In case of experimental data, the last field reports the variance of the spatial distribution observed for the associated locus.