丝状真菌基因编辑

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丝状真菌在工业、农业和医药领域至关重要的多细胞微生物，普因特生物以CRISPR/Cas9系统为代表的第三代编辑技术，凭借其高效性和简便性可对丝状真菌基因组进行定点修饰，包括敲除特定基因、敲入外源序列、引入点突变等，以实现菌株功能优化和代谢通路重构。

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▶经验丰富：在丝状真菌基因编辑领域有丰富的经验积累，经验丰富的团队可以帮助您快速完成丝状真菌突变体构建；

▶质量保证：确保所有基因编辑位点都经过测序鉴定，以确保产品质量。

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The role of the Flb protein family in the life cycle of Aspergillus niger

期刊：Antonie van Leeuwenhoek

Growth of A. niger MA234.1 and the ∆flbA, ∆flbB, ∆flbC, ∆flbD and ∆flbE strains on PDA and on MMA with different carbon sources (A), as well as biomass (B), number of spores (C), and number of spores per mg mycelium (D) of cultures grown on MMA with glucose (MMA-G). Cultures were grown for 5 (A) and 7 (B) days from a point inoculum or grown for 3 days after confluent spreading of spores on a plate(C, D). To determine biomass, colonies were grown in between two perforated polycarbonate membranes, while spores were harvested from colonies that had grown on MMA-G in the absence of the membranes. Statistical analysis was done with One-way ANOVA with different letters indicating statistical differences.

适用领域

▶提升工业酶与化学品产量：通过改造生产酶制剂、有机酸的真菌工业底盘（如黑曲霉、灰葡萄孢霉），来优化代谢网络，显著提高目标产物产量。

▶开发新型药物：激活或敲除真菌体内的沉默基因簇（BGCs），以发现和高效生产具有抗菌、抗癌等活性的新型天然产物。

▶改良食用菌与作物病害防控：编辑香菇基因以提升生物质转化率，编辑灵芝基因实现三萜类药用成分产量翻倍；同时，通过敲除稻瘟病菌、尖孢镰刀菌等病原菌的致病基因，为开发绿色防控策略提供新靶点。

▶解析基因功能：构建基因敲除或突变菌株，结合多组学分析，从全局角度阐明基因调控网络和代谢途径。