解脂耶氏酵母基因编辑

服务介绍

解脂耶氏酵母（Yarrowia lipolytica）是一种极具潜力的工业微生物底盘，以其独特的油脂代谢能力和强大的遗传可塑性而著称。普因特生物以CRISPR/Cas9系统为代表的第三代编辑技术，凭借其高效性和简便性可对解脂耶氏酵母基因组进行定点修饰，包括敲除特定基因、敲入外源序列、引入点突变等，以实现菌株功能优化和代谢通路重构。

服务优势

▶经验丰富：在解脂耶氏酵母基因编辑领域有丰富的经验积累，经验丰富的团队可以帮助您快速完成解脂耶氏酵母突变体构建；

▶质量保证：确保所有基因编辑位点都经过测序鉴定，以确保产品质量。

服务流程

文案展示

Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica

期刊：Engineering Microbiology

Optimization of the CRISPR/Cas9 system for improved gene disruption efficiency.(A)Schematic of different promoters of sgRNA used in this study.sgRNA is a single guide RNA,HH is the hammerhead ribozyme,and HDV is the hepatitis delta virus ribozyme.Synthetic RNA polymerase (Pol)III promoters were generated by inserting the SNR52p or SCR1p sequences immediately upstream of tRNAGly.Polyt,which is a string of four thymines,served as the Pol III terminator. (B)URA3 disruption efficiencies achieved with different sgRNA promoters in the PO1f-Cas9-URA3 strain.(C)Validation of the efficiency of URA3 gene disruption. Transformants carrying different sgRNA promoters were randomly selected and streaked onto YPD and SC-URA plates,respectively.In total,20 colonies from each biological parallel were picked and tested to calculate the gene disruption efficiency of the system.(D)URA3 disruption efficiency under different Cas9 expression conditions.

适用领域

▶代谢工程与化学品生产：通过敲除竞争途径、过表达关键酶，可高效生产用于航空燃油的脂肪酸乙酯、中链脂肪酸以及微生物油脂。

▶功能基因组学与细胞工厂优化：系统性敲除核心代谢基因，有助于从分子层面阐明脂质积累等复杂代谢机制。

▶蛋白质/酶的生产与改造：针对重组蛋白产量低、易降解的行业难题，基因编辑能提供有效解决方案。

▶工业底盘细胞构建：通过删除细菌载体元件、抗生素抗性基因以及构建多重营养缺陷型标记，可开发出符合工业安全和生物防护标准的新一代底盘菌株。