乳酸菌基因编辑

服务介绍

乳酸菌（Lactic Acid Bacteria, LAB）是一类能够发酵糖类、主要产物为乳酸的人体和食品工业极为有益的微生物，兼具安全、易培养、代谢多样等优点。普因特生物以CRISPR/Cas9系统为代表的第三代编辑技术，凭借其高效性和简便性可对乳酸菌基因组进行定点修饰，包括敲除特定基因、敲入外源序列、引入点突变等，以实现菌株功能优化和代谢通路重构。

服务优势

▶经验丰富：在乳酸菌基因编辑领域有丰富的经验积累，经验丰富的团队可以帮助您快速完成乳酸菌突变体构建；

▶质量保证：确保所有基因编辑位点都经过测序鉴定，以确保产品质量。

服务流程

文案展示

lopment of a RecE/T‐Assisted CRISPR–Cas9 Toolbox for Lactobacillus

期刊：Biotechnology Journal

Overview of CRISPR–Cas9 editing toolbox for Lactobacilli. The CRISPR–Cas9 editing toolbox consists of three pathways for genome editing in Lactobacillus.“ Route 1” represents the all‐in‐one editing plasmid (pHS series) strategy applicable for the species,  in which endogenous recombination machinery is qualified for DSB repair.“ Route 2” represents the association of the all‐in‐one editing plasmid (pHS series) and RecE/T ‐ based assistant plasmid (pLH series) strategy, applicable for the species in which endogenous recombination is deficient in DSB repair. The RecE/T recombinases mediate HDR of DSB by employing homologous arms provided by the editing plasmid.“ Route 3” based on nickase‐induced chro mosomal single‐strand break (nick), is an alternative strategy applicable for the species in which the chromosomal nick is able to effectively stimulate HDR, realizing genetic modification.

适用领域

▶基础科学研究：可用于特定基因功能研究、代谢通路解析与优化，或使菌株携带荧光标记便于体内追踪。

▶益生菌与医药开发：用于开发疗效更优的下一代益生菌，或将其改造为递送疫苗、抗体等生物活性分子的“活体药物”载体。

▶食品工业：通过优化代谢通路提高目标产物（如γ-氨基丁酸、维生素）产量，或去除产生异味、影响风味的基因，改善发酵食品品质。